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New Users Guide

New users should contact Deniz Ucar at ducar@tulane.edu, and provide the following:

  • Contact Information
  • Research Lab and Primary Investigator
  • Your Position
  • Brief Description of Your Imaging Needs

Each piece of equipment requires separate training regardless of previous experience and must come from the Core Personnel. 

After completing the training, users will receive an access code to the Brain Institute Cell and Tissue Imaging Core and equipment and will be able to schedule sessions.  

Rates

Before training can begin on the Olympus Microscope, Zeiss Slide Scanner, or Leica LMD system, users will need to complete the Account Authorization Form and have it signed by the Primary Investigator. 

Account Authorization Form

Olympus Microscope
Usage: $20 per hour
Training: $30 per hour

Zeiss Slide Scanner
Usage: $10 per hour
Training: $20 per hour

Leica LMD7
Usage: FREE
Training: $20 per hour

If you need help with imaging, please fill out the Service Request Form and contact Deniz Ucar at ducar@tulane.edu

 

Users of the Olympus Microscope or Zeiss Slide Scanner or Leica LMD7 must name their files in the following standard format:

(YearMonthDay_FileName)
Example:  2019May25_test.vsi

Each user must create a new folder (with name of user) within their lab folder (named for the primary investigator) and save data.  Data will be stored on the hard drive in the Brain Institute Cell and Tissue Imaging Core for a period of one month.  

Once per week, the technician will clear files that have been stored for one month from the hard drive.  Prior to deletion of files, primary investigators and the user who acquired the data will be notified via email that their data will be deleted in one week.  At the end of the one month storage period, responsibility for data management falls exclusively to the investigators.

Protocols

Leica LMD7- Laser Micro Dissection System

Leica_LMD-Protocol_SamplePrep

Leica-LMD7-Paraffin or Frozen tissue protocol

Leica LMD Remote Training Viedo

LMD7_Quick Start Guide

Olympus FV3000 Confocal Training Videos

Feedback and Acknowledgments

We welcome any feedback from users of the Brain Institute Cell and Tissue Imaging Core.  Please email any comments or suggestions to Dr. Jill Daniel jmdaniel@tulane.edu.

All users of the Olympus Confocal Microscope in the Brain Institute Cell and Tissue Imaging Core are asked to acknowledge the grant under which the microscope was purchased in their paper as the “Louisiana Board of Regents Departmental Enhancement Grant LEQSF(2018-23)-ENH-DE-15.”  Please send a copy of the paper to Dr. Jill Daniel, Brain Institute Director, at jmdaniel@tulane.edu.